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概要
Trypsin-EDTA (0.05%) can be used to achieve a single-cell suspension of human embryonic stem (ES) cells or human induced pluripotent stem (iPS) cells for flow cytometry or cloning assays. This is a gentler version than the standard concentration (0.25%), and has applications to many other cell culture systems in addition to human ES cells and iPS cells.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Trypsin-EDTA (0.05%) | 07910 | All | English |
| Safety Data Sheet 1 | Trypsin-EDTA (0.05%) | 07910 | All | English |
| Safety Data Sheet 2 | Trypsin-EDTA (0.05%) | 07910 | All | English |
数据及文献
Publications (1)
Nature Communications 2015 DEC
Intermediate DNA methylation is a conserved signature of genome regulation
Abstract
Abstract
The role of intermediate methylation states in DNA is unclear. Here, to comprehensively identify regions of intermediate methylation and their quantitative relationship with gene activity, we apply integrative and comparative epigenomics to 25 human primary cell and tissue samples. We report 18,452 intermediate methylation regions located near 36% of genes and enriched at enhancers, exons and DNase I hypersensitivity sites. Intermediate methylation regions average 57% methylation, are predominantly allele-independent and are conserved across individuals and between mouse and human, suggesting a conserved function. These regions have an intermediate level of active chromatin marks and their associated genes have intermediate transcriptional activity. Exonic intermediate methylation correlates with exon inclusion at a level between that of fully methylated and unmethylated exons, highlighting gene context-dependent functions. We conclude that intermediate DNA methylation is a conserved signature of gene regulation and exon usage.
