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EasyPlate™ EasySep™ Magnet

Magnet for column-free immunomagnetic separation

概要
技术资料
数据及文献

概要

The EasyPlate™ EasySep™ Magnet is designed for cell separation procedures using selected EasySep™ reagents when processing a small number of cells (up to 2 x 10^7 cells per well). The EasyPlate™ EasySep™ Magnet generates a high-gradient magnetic field that is strong enough to separate cells labeled with EasySep™ D Magnetic Particles without the use of columns. The EasyPlate™ EasySep™ Magnet is designed to hold a round-bottom non-tissue culture-treated 96-well plate.

技术资料

Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet EasyPlate™ EasySep™ Magnet 18102 All English

数据及文献

Publications (1)

Blood 2014 OCT Human CD68 promoter GFP transgenic mice allow analysis of monocyte to macrophage differentiation in vivo. Iqbal AJ et al.

Abstract

The recruitment of monocytes and their differentiation into macrophages at sites of inflammation are key events in determining the outcome of the inflammatory response and initiating the return to tissue homeostasis. To study monocyte trafficking and macrophage differentiation in vivo, we have generated a novel transgenic reporter mouse expressing a green fluorescent protein (GFP) under the control of the human CD68 promoter. CD68-GFP mice express high levels of GFP in both monocyte and embryo-derived tissue resident macrophages in adult animals. The human CD68 promoter drives GFP expression in all CD115(+) monocytes of adult blood, spleen, and bone marrow; we took advantage of this to directly compare the trafficking of bone marrow-derived CD68-GFP monocytes to that of CX3CR1(GFP) monocytes in vivo using a sterile zymosan peritonitis model. Unlike CX3CR1(GFP) monocytes, which downregulate GFP expression on differentiation into macrophages in this model, CD68-GFP monocytes retain high-level GFP expression for 72 hours after differentiation into macrophages, allowing continued cell tracking during resolution of inflammation. In summary, this novel CD68-GFP transgenic reporter mouse line represents a powerful resource for analyzing monocyte mobilization and monocyte trafficking as well as studying the fate of recruited monocytes in models of acute and chronic inflammation.
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