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ArciTect™ Cas9 Nuclease

Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing

概要
技术资料
数据及文献

概要

ArciTect™ Cas9 Nuclease requires association with a guide RNA—e.g. ArciTect™ sgRNA (Catalog #200-0013) or a duplex composed of ArciTect™ tracrRNA (Catalog #76016) and ArciTect™ crRNA (Catalog #76010)—to form a ribonucleoprotein (RNP) complex. This RNP complex then creates double-strand breaks at site-specific locations in the genome. ArciTect™ Cas9 Nuclease contains a nuclear localization signal at the N-terminus, ensuring that the RNP complex translocates to the nucleus, thereby increasing the efficiency of genome editing. As the RNP complex is fully functional upon transfection, it allows for immediate activity following translocation to the nucleus. The RNP complex is degraded over 48 hours, allowing sufficient time for genome editing to occur while reducing off-target effects that can be caused by the continuous presence of the RNP complex. Using the RNP system also circumvents the laborious process of generating stable Cas9-expressing cell lines, saving time and reducing the risk of off-target effects due to leaky inducible expression systems. The S. pyogenes Cas9 uses the protospacer adjacent motif (PAM) sequence NGG (where N can be any nucleotide). The enzyme will not cleave without a genomic PAM site downstream of the target sequence.

技术资料

Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet ArciTect™ Cas9 Nuclease 76002, 76004 All English
Special Protocol ArciTect™ Cas9 Nuclease 76001 All English
Safety Data Sheet ArciTect™ Cas9 Nuclease 76002, 76004 All English

数据及文献