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概要
The Mouse Interleukin 12 (IL-12) p70 ELISA Kit is designed for the quantitative detection and measurement of mouse IL-12 (p70) in biological fluids such as serum, plasma, and cell culture supernatants. IL-12 (p70) is a heterodimeric cytokine composed of p35 and p40 subunits; the p40 subunit is shared with IL-23. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells. IL-12 is a regulator of cytokine synthesis, especially IFN-γ, enhances the proliferation of T and NK cells, and stimulates the differentiation of CD4+ and CD8+ T cells.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Mouse IL-12 (p70) ELISA Kit | 02026, 02027 | All | English |
| Safety Data Sheet 1 | Mouse IL-12 (p70) ELISA Kit | 02026, 02027 | All | English |
| Safety Data Sheet 2 | Mouse IL-12 (p70) ELISA Kit | 02026, 02027 | All | English |
| Safety Data Sheet 3 | Mouse IL-12 (p70) ELISA Kit | 02026, 02027 | All | English |
| Safety Data Sheet 4 | Mouse IL-12 (p70) ELISA Kit | 02026 | All | English |
| Safety Data Sheet 5 | Mouse IL-12 (p70) ELISA Kit | 02026, 02027 | All | English |
| Safety Data Sheet 6 | Mouse IL-12 (p70) ELISA Kit | 02026, 02027 | All | English |
| Safety Data Sheet 7 | Mouse IL-12 (p70) ELISA Kit | 02026, 02027 | All | English |
| Safety Data Sheet 8 | Mouse IL-12 (p70) ELISA Kit | 02026, 02027 | All | English |
| Safety Data Sheet 9 | Mouse IL-12 (p70) ELISA Kit | 02026, 02027 | All | English |
| Safety Data Sheet 10 | Mouse IL-12 (p70) ELISA Kit | 02026, 02027 | All | English |
| Safety Data Sheet 1 | Mouse IL-12 (p70) ELISA Kit | 02027 | All | English |
数据及文献
Data
Representative Standard Curve
• Reportable range: 10 - 3160 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 6 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: No international standard exists for calibration.
• Recovery: A mid-curve recovery of 85 - 121% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.7% (CV). The inter-assay precision of this assay is 6.8% (CV).
