Mouse IL-4 ELISA Kit_产品中心_北京砺达辰生物科技有限公司

ELISAs

Mouse IL-4 ELISA Kit

For detection and measurement of mouse interleukin 4

概要

技术资料

数据及文献

概要

The Mouse Interleukin 4 (IL-4) ELISA Kit is designed for the quantitative detection and measurement of mouse IL-4 in biological fluids such as serum, plasma, and cell culture supernatants. IL-4 is produced by Th2 cells, NK cells, γ/δ T cells, eosinophils, mast cells, and activated basophils. IL-4 induces Ig isotype switching in B cells, regulates the differentiation of naïve T cells into Th2 cells, and in conjunction with TNF, recruits T cells and eosinophils to sites of inflammation.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Mouse IL-4 ELISA Kit	02038, 02039	All	English
Safety Data Sheet 1	Mouse IL-4 ELISA Kit	02038, 02039	All	English
Safety Data Sheet 2	Mouse IL-4 ELISA Kit	02038, 02039	All	English
Safety Data Sheet 3	Mouse IL-4 ELISA Kit	02038, 02039	All	English
Safety Data Sheet 4	Mouse IL-4 ELISA Kit	02038, 02039	All	English
Safety Data Sheet 5	Mouse IL-4 ELISA Kit	02038, 02039	All	English
Safety Data Sheet 6	Mouse IL-4 ELISA Kit	02038, 02039	All	English
Safety Data Sheet 7	Mouse IL-4 ELISA Kit	02038, 02039	All	English
Safety Data Sheet 8	Mouse IL-4 ELISA Kit	02038, 02039	All	English
Safety Data Sheet 9	Mouse IL-4 ELISA Kit	02038, 02039	All	English
Safety Data Sheet 10	Mouse IL-4 ELISA Kit	02038, 02039	All	English
Safety Data Sheet 11	Mouse IL-4 ELISA Kit	02038, 02039	All	English

数据及文献

Data

Representative Standard Curve

• Reportable Range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 2 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 91/656.
• Recovery: A mid-curve recovery of 94 - 103% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.9% (CV). The inter-assay precision of this assay is 3.9% (CV).