Human pan IFN-alpha ELISA Kit_产品中心_北京砺达辰生物科技有限公司

ELISAs

Human pan IFN-alpha ELISA Kit

For detection and measurement of human pan interferon-alpha

概要

技术资料

数据及文献

概要

The Human pan Interferon-alpha (IFN-α) ELISA Kit is designed for the quantitative detection and measurement of human IFN-α in biological fluids such as serum, plasma, and cell culture supernatants. IFN-α is primarily produced by macrophages resulting from viral infections and activates anti-viral and immunomodulatory activities in target cells. IFN-α is secreted as multiple subtypes; this kit is specific for subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16, and 17.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human pan IFN-alpha ELISA Kit	02000, 02001	All	English
Safety Data Sheet 1	Human pan IFN-alpha ELISA Kit	02000, 02001	All	English
Safety Data Sheet 2	Human pan IFN-alpha ELISA Kit	02000, 02001	All	English
Safety Data Sheet 3	Human pan IFN-alpha ELISA Kit	02000, 02001	All	English
Safety Data Sheet 4	Human pan IFN-alpha ELISA Kit	02000, 02001	All	English
Safety Data Sheet 5	Human pan IFN-alpha ELISA Kit	02000, 02001	All	English
Safety Data Sheet 6	Human pan IFN-alpha ELISA Kit	02000	All	English
Safety Data Sheet 7	Human pan IFN-alpha ELISA Kit	02000, 02001	All	English
Safety Data Sheet 8	Human pan IFN-alpha ELISA Kit	02000, 02001	All	English
Safety Data Sheet 9	Human pan IFN-alpha ELISA Kit	02000, 02001	All	English
Safety Data Sheet 10	Human pan IFN-alpha ELISA Kit	02000, 02001	All	English
Safety Data Sheet 11	Human pan IFN-alpha ELISA Kit	02000, 02001	All	English

数据及文献

Data

Representative Standard Curve

• Reportable range: 3.2 - 316 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC* international standard 95/566.
• Recovery: A mid-curve recovery of 92 - 123% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.7% (CV). The inter-assay precision of this assay is 10.0% (CV).

*National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.