Human IL-17A ELISA Kit_产品中心_北京砺达辰生物科技有限公司

ELISAs

Human IL-17A ELISA Kit

For detection and measurement of human interleukin 17A

概要

技术资料

数据及文献

概要

The Human Interleukin 17A (IL-17A) ELISA Kit is designed for the quantitative detection and measurement of human IL-17A in biological fluids such as serum, plasma, and cell culture supernatants. IL-17A is secreted by Th17 cells, CD8+ T cells, γδ T cells, natural killer T cells, B cells, innate lymphoid cells, and mesenchymal stromal cells. IL-17A is a pro-inflammatory cytokine that plays a key role in the defense against pathogens, activates the NF-kB, MAPK, and C/EBP pathways, and in conjunction with TNF-α, induces sustained neutrophil recruitment during inflammation.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human IL-17A ELISA Kit	02036, 02037	All	English
Safety Data Sheet 1	Human IL-17A ELISA Kit	02036, 02037	All	English
Safety Data Sheet 2	Human IL-17A ELISA Kit	02036, 02037	All	English
Safety Data Sheet 3	Human IL-17A ELISA Kit	02036, 02037	All	English
Safety Data Sheet 4	Human IL-17A ELISA Kit	02036, 02037	All	English
Safety Data Sheet 5	Human IL-17A ELISA Kit	02036, 02037	All	English
Safety Data Sheet 6	Human IL-17A ELISA Kit	02036, 02037	All	English
Safety Data Sheet 7	Human IL-17A ELISA Kit	02036, 02037	All	English
Safety Data Sheet 8	Human IL-17A ELISA Kit	02036, 02037	All	English
Safety Data Sheet 9	Human IL-17A ELISA Kit	02036, 02037	All	English
Safety Data Sheet 10	Human IL-17A ELISA Kit	02036, 02037	All	English

数据及文献

Data

Representative Standard Curve

• Reportable Range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 01/420.
• Recovery: A mid-curve recovery of 96 - 119% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.9% (CV). The inter-assay precision of this assay is 7.3% (CV).