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概要
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human IL-1 beta ELISA Kit | 02004, 02005 | All | English |
| Safety Data Sheet 1 | Human IL-1 beta ELISA Kit | 02004, 02005 | All | English |
| Safety Data Sheet 2 | Human IL-1 beta ELISA Kit | 02004 | All | English |
| Safety Data Sheet 3 | Human IL-1 beta ELISA Kit | 02004, 02005 | All | English |
| Safety Data Sheet 4 | Human IL-1 beta ELISA Kit | 02004, 02005 | All | English |
| Safety Data Sheet 5 | Human IL-1 beta ELISA Kit | 02004, 02005 | All | English |
| Safety Data Sheet 6 | Human IL-1 beta ELISA Kit | 02004, 02005 | All | English |
| Safety Data Sheet 7 | Human IL-1 beta ELISA Kit | 02004, 02005 | All | English |
| Safety Data Sheet 8 | Human IL-1 beta ELISA Kit | 02004, 02005 | All | English |
| Safety Data Sheet 9 | Human IL-1 beta ELISA Kit | 02004 | All | English |
| Safety Data Sheet 10 | Human IL-1 beta ELISA Kit | 02004, 02005 | All | English |
| Safety Data Sheet 1 | Human IL-1 beta ELISA Kit | 02005 | All | English |
| Safety Data Sheet 2 | Human IL-1 beta ELISA Kit | 02005 | All | English |
数据及文献
Data
Representative Standard Curve
• Reportable Range: 1 - 316 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 4 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 86/680.
• Recovery: A mid-curve recovery of 83 - 98% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.6% (CV). The inter-assay precision of this assay is 3.6% (CV).
