Mouse TNF-alpha ELISA Kit_产品中心_北京砺达辰生物科技有限公司

ELISAs

Mouse TNF-alpha ELISA Kit

For detection and measurement of mouse tumor necrosis factor-alpha

概要

技术资料

数据及文献

概要

The Mouse Tumor Necrosis Factor-alpha (TNF-α) ELISA Kit is designed for the quantitative detection and measurement of mouse TNF-α in biological fluids such as serum, plasma, and cell culture supernatants. TNF-α is primarily produced by activated T cells and macrophages in response to inflammation or infection, but is also produced by B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α is a pro-inflammatory cytokine and stimulates anti-tumor responses in vitro.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Mouse TNF-alpha ELISA Kit	02030, 02031	All	English
Safety Data Sheet 1	Mouse TNF-alpha ELISA Kit	02030, 02031	All	English
Safety Data Sheet 2	Mouse TNF-alpha ELISA Kit	02030, 02031	All	English
Safety Data Sheet 3	Mouse TNF-alpha ELISA Kit	02030, 02031	All	English
Safety Data Sheet 4	Mouse TNF-alpha ELISA Kit	02030, 02031	All	English
Safety Data Sheet 5	Mouse TNF-alpha ELISA Kit	02030, 02031	All	English
Safety Data Sheet 6	Mouse TNF-alpha ELISA Kit	02030, 02031	All	English
Safety Data Sheet 7	Mouse TNF-alpha ELISA Kit	02030	All	English
Safety Data Sheet 8	Mouse TNF-alpha ELISA Kit	02030, 02031	All	English
Safety Data Sheet 9	Mouse TNF-alpha ELISA Kit	02030, 02031	All	English
Safety Data Sheet 10	Mouse TNF-alpha ELISA Kit	02030, 02031	All	English
Safety Data Sheet 1	Mouse TNF-alpha ELISA Kit	02031	All	English

数据及文献

Data

Representative Standard Curve

• Reportable range: 15.6 - 2000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 5 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 88/532.
• Recovery: A mid-curve recovery of 84 - 86% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.4% (CV). The Inter-assay precision of this assay is 3.7% (CV).

* National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.