Human IFN-gamma ELISA Kit_产品中心_北京砺达辰生物科技有限公司

ELISAs

Human IFN-gamma ELISA Kit

For detection and measurement of human interferon-gamma

概要

技术资料

数据及文献

概要

The Human Interferon-gamma (IFN-γ) ELISA Kit is designed for the quantitative detection and measurement of human IFN-γ in biological fluids such as serum, plasma, and cell culture supernatants. IFN-γ is primarily secreted by T and NK cells, but is also produced by dendritic cells and macrophages in smaller amounts. IFN-γ is a pleiotropic cytokine that modulates a broad range of immunological functions including antimicrobial and antitumor activity, and increases the surface expression of MHC class I and II proteins.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human IFN-gamma ELISA Kit	02002, 02003	All	English
Safety Data Sheet 1	Human IFN-gamma ELISA Kit	02002, 02003	All	English
Safety Data Sheet 2	Human IFN-gamma ELISA Kit	02002, 02003	All	English
Safety Data Sheet 3	Human IFN-gamma ELISA Kit	02002, 02003	All	English
Safety Data Sheet 4	Human IFN-gamma ELISA Kit	02002, 02003	All	English
Safety Data Sheet 5	Human IFN-gamma ELISA Kit	02002, 02003	All	English
Safety Data Sheet 6	Human IFN-gamma ELISA Kit	02002	All	English
Safety Data Sheet 7	Human IFN-gamma ELISA Kit	02002, 02003	All	English
Safety Data Sheet 8	Human IFN-gamma ELISA Kit	02002, 02003	All	English
Safety Data Sheet 9	Human IFN-gamma ELISA Kit	02002, 02003	All	English
Safety Data Sheet 10	Human IFN-gamma ELISA Kit	02002, 02003	All	English
Safety Data Sheet 1	Human IFN-gamma ELISA Kit	02003	All	English

数据及文献

Data

Representative Standard Curve

• Reportable range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIAID* international standard Gxg01-902-535.
• Recovery: A mid-curve recovery of 85 - 91% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.9% (CV). The inter-assay precision of this assay is 4.4% (CV).

*National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892-6612, USA.