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概要
Neuroblastoma overexpressed gene protein (NOV) is a member of the CCN (CYR61/CTGF/NOV) family. NOV contains four structural domains: a non-functional N-terminal insulin-like growth factor-binding protein (IGFBP) domain, a von Willebrand factor C (vWFC) domain that mediates oligomerization, a thrombospondin type I domain that mediates matrix interactions, and a C-terminal cysteine knot domain that interacts with fibulin 1C, Notch1, and CCN2 (Perbal 2004; Perbal 2006; Perbal et al.; Sakamoto et al.). NOV interacts with integrins αVβ3 and α5β1 to mediate adhesion of endothelial cells, chemotaxis, and angiogenesis (Lin et al.). It also interacts with gap junction protein connexin43 to suppress cell proliferation (Fu et al.). During development, NOV is expressed in multiple tissues including: muscle, endothelium, nervous system, adrenal cortex, and chondrocytes (Martinerie et al.; Perbal 2004; Perbal 2006; Snaith et al.). In cancers expressing the N-terminally truncated version, NOV localizes to the nucleus and promotes proliferation (Planque et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant NOV | 78198, 78198.1 | All | English |
| Safety Data Sheet | Human Recombinant NOV | 78198, 78198.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant NOV was tested by its ability to promote the proliferation of 3T3 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the protein at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 5 μg/mL. (B) 2 μg of Human Recombinant NOV was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant NOV has a predicted molecular mass of 35.7 kDa.
