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概要
Nerve growth factor (NGF)-beta is a prototypical member of the neurotrophin family and has a role in the survival and growth of neural cells, regulating cell growth, promoting differentiation into neurons, and neuron migration. The beta subtype of NGF is biologically active in comparison to the alpha-2 and gamma-2 subtypes. NGF-beta in its secreted form can bind to tyrosine kinase A (TrkA) receptor with high affinity and to p75 (NTR) with low affinity (Levi & Alemà; Sofroniew et al.). NGF has been shown to possess pro-inflammatory and pro-fibrogenic properties (Micera et al.). It has also been shown that overexpression of NGF-beta promotes differentiation of bone marrow mesenchymal stem cells into neurons through regulation of AKT and MAPK pathways (Yuan et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant NGF-beta | 78092, 78092.1 | All | English |
| Safety Data Sheet | Human Recombinant NGF-beta | 78092, 78092.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant NGF-beta was tested by its ability to promote the proliferation of TF-1 cells grown in GM-CSF free medium. Cell proliferation was measured after 67 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.54 ng/mL. (B) 1 μg of Human Recombinant NGF-beta was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant NGF-beta is a homodimer of 13.6 kDa subunits with a predicted total molecular mass of 27.3 kDa.
