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概要
Macrophage inflammatory protein-3 alpha (MIP-3 alpha) or CCL20 is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils. MIP-3 alpha has been shown to be expressed predominantly in liver, lymph nodes, appendix, peripheral blood lymphocytes, and lungs, and its expression is markedly upregulated by mediators of inflammation such as tumor necrosis factor or lipopolysaccharide (Hromas et al.). MIP-3 alpha signals through the CCR6 receptor, a chemokine receptor that is selectively and highly expressed in human CD34+ cell-derived dendritic cells (Baba et al.; Greaves et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant MIP-3 alpha (CCL20) | 78118, 78118.1 | All | English |
| Safety Data Sheet | Human Recombinant MIP-3 alpha (CCL20) | 78118, 78118.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant MIP-3 alpha (CCL20) was tested by its ability to mobilize Ca2+ in CHO-K1/Gα15/hCCR6 cells (human Gα15 and human CCR6 stably expressed in CHO-K1 cells). Ca2+ mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the above example is less than 0.2 μg/mL. (B) 2 μg of Human Recombinant MIP-3 alpha (CCL20) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.
