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概要
Monocyte chemotactic protein-1 (MCP-1), also known as CCL2, is a member of the CC family of chemokines. The protein is primarily induced by platelet-derived growth factor (PDGF) gene (Cochran et al.). The biological effects of MCP-1 are mediated via the specific G-protein-coupled receptor CCR2 which in turn activates signal transduction pathways leading to monocyte transmigration (Sozzani et al.). Migration of monocytes from the bloodstream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as other immunomodulatory effects. MCP-1 is produced by a variety of cell types, including fibroblasts and endothelial, epithelial, smooth muscle, mesangial, astrocytic, monocytic, and microglial cells, which are important for antiviral responses in the peripheral circulations and in tissues (Cushing et al.; Deshmane et al.). MCP-1 plays a role in physiological processes such as neurogenesis, neuroprotection, and neurotransmission and has important implications in neurological disorders such as multiple sclerosis and Alzheimer’s disease, in which it is produced during neuroinflammation at the sites of lesions (Conductier et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant MCP-1 (CCL2) | 78087, 78087.1 | All | English |
| Safety Data Sheet | Human Recombinant MCP-1 (CCL2) | 78087.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant MCP-1 was tested by its ability to induce chemotaxis of THP-1 cells. Cell migration was measured after 1 hour using a fluorometric assay method. Increase in migration over basal level was seen starting at 1 ng/mL. (B) 1 μg of Human Recombinant MCP-1 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant MCP-1 has a predicted molecular mass of 8.7 kDa.
