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概要
Interferon gamma-inducible protein (IP) 10 or CXCL10 is a member of the CXC chemokine family. It binds CXCR3 activating ERK1/2, p38/MAPK, JNK, and PI3-kinase/AKT signaling pathways, inducing intracellular calcium influx, DNA synthesis, cell proliferation, and chemotaxis. IP-10 regulates innate and adaptive immune responses by affecting the function of activated T cells, natural killer cells, inflammatory dendritic cells, macrophages, and B cells. IP-10 is produced by leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, fibroblasts, and keratinocytes in response to IFN-gamma. IP-10 has been implicated in a wide range of diseases, including infectious diseases and chronic inflammatory and autoimmune diseases, as well as in tumor formation (Liu et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IP-10 (CXCL10) | 78165, 78165.1 | All | English |
| Safety Data Sheet | Human Recombinant IP-10 (CXCL10) | 78165, 78165.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IP-10 (CXCL10) was tested using a Ca2+ mobilization assay in CHO-K1 stably expressing Gα15 and CXCR3. Calcium mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which calcium mobilization is at 50% of maximum. The EC50 in the example above is less than 0.2 μg/mL. (B) 2 μg of Human Recombinant IP-10 (CXCL10) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IP-10 (CXCL10) has a predicted molecular mass of 8.8 kDa.
