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概要
Interleukin 33 (IL-33) is a pro-inflammatory cytokine from the IL-1 family. It binds to ST2 receptor and activates NF-κB and MAPK pathways. IL-33 is expressed by epithelial cells, smooth muscle cells, and fibroblasts in various tissues and organs, as well as resting basophils, mast cells, eosinophils, natural helper cells, group 2 innate lymphoid cells, dendritic cells, and activated macrophages (Schmitz et al.; Yasuda et al.). It contributes to allergic inflammation by stimulating production of the cytokines IL-4, IL-5, and IL-13, and stimulates host defense against microbial and viral infections (Liew; Yasuda et al.). In the central nervous system, IL-33 is produced by endothelial cells and astrocytes. It induces proliferation of microglia and mediates production of pro-inflammatory cytokines (Yasuoka et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IL-33 | 78043, 78043.1 | All | English |
| Safety Data Sheet | Human Recombinant IL-33 | 78043, 78043.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IL-33 was tested by its ability to promote the proliferation of D10S cells in low serum
medium containing no other cytokines. Cell proliferation was measured after 67 hours of culture using a fluorometric assay method. The
EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the
above example is 0.1 - 0.15 ng/mL.
(B) 1 μg of Human Recombinant IL-33 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized
by Coomassie Blue staining. Human Recombinant IL-33 has a predicted molecular mass of 18.1 kDa.
