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概要
Interleukin 15 (IL-15) is a four-alpha helix bundle cytokine with many similar properties to IL-2, with which it shares components of its receptor. The IL-15 receptor is a heterotrimeric receptor composed of IL-15Ra, the high-affinity receptor for IL-15, as well as IL-2/15Rb (CD122) and common gamma chain (CD132). IL-15 binds to IL-15Rα receptor and can then be presented in trans to IL-2/15Rb and common gamma chain on other cells. Trans-presentation is thought to be the major mechanism by which IL-15-mediated responses occur in mice, although may not be necessary in humans (Castillo et al.). The cytoplasmic domains of IL-2/15Rb and common gamma chain mediate signaling to activate JAK/STAT and PI3K pathways. IL-15 supports the survival and proliferation of naive CD4+ and CD8+ T cells, and promotes homeostasis of memory T cells. IL-15 also promotes the survival and differentiation of NK cells and regulates their cytolytic activity (Ma et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IL-15 | 78031, 78031.1, 78031.2, 78031.3 | All | English |
| Safety Data Sheet | Human Recombinant IL-15 | 78031, 78031.1, 78031.2, 78031.3 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IL-15 was tested by its ability to promote the proliferation of CTLL-2 cells growing in the
presence of other cytokines and stimuli. Cell proliferation was measured after 65 hours of culture using a fluorometric assay method. The
EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the
above example is 0.13 - 0.19 ng/mL.
(B) 1 ug of Human Recombinant IL-15 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized
by Coomassie Blue staining. Human Recombinant IL-15 has a predicted molecular mass of 12.9 kDa.
