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概要
Interleukin 10 (IL-10) is the founding member of the IL-10 family of class II cytokines. All of the IL-10 cytokine family members have a four-helix bundle consisting of α-helical folds. Upon binding to its receptor, IL-10 activates signaling through JAK1 and STAT3. It is produced by dendritic cells, macrophages, and CD4+ T cells, as well as mast cells, NK cells, neutrophils, and B cells, under specific stimulating conditions (Saraiva & O'Garra). IL-10 can inhibit the expression of pro-inflammatory cytokines and promote healing processes, and is important for the function of regulatory T cells. IL-10 also enhances B cell proliferation, immunoglobulin secretion, and class II MHC expression, while IL-10 produced by macrophages inhibits activation of neighboring macrophages, thus allowing a level of self-regulation (Ouyang et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IL-10 | 78024, 78024.1, 78024.2 | All | English |
| Safety Data Sheet | Human Recombinant IL-10 | 78024, 78024.1, 78024.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IL-10 was tested by its ability to promote the proliferation of MC/9 cells. Cell
proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at
which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 0.2 ng/mL.
(B) 5 μg of Human Recombinant IL-10 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized
by Coomassie Blue staining. Human Recombinant IL-10 has a predicted molecular mass of 18 - 19 kDa.
