网站首页
概要
Interleukin 7 (IL-7) is a member of the Type I cytokine family that is critical for T and B cell development and survival. It is produced by non-hematopoietic cells in the thymus, lymphoid organs, and by bone marrow stromal cells (Lundström et al.). IL-7 binds to a receptor (IL-7R) composed of common gamma chain and IL-7Ra (CD127) and signals through the JAK/STAT and PI3K pathways. IL-7 regulates the survival of naïve and memory CD4+ and CD8+ T cells, γδ T cells, NK T cells, innate lymphoid cells, and T regulatory cells (Carrette & Surh). Although a deficiency in IL-7R still permits the generation of normal numbers of peripheral B cells in humans, stimulation of human B cell precursors with IL-7 could promote STAT-5-dependent proliferation and survival in vitro (Clark et al.; Corfe & Paige).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IL-7 | 78053, 78053.1, 78053.2, 78053.3 | All | English |
| Safety Data Sheet | Human Recombinant IL-7 | 78053, 78053.1, 78053.2, 78053.3 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IL-7 was tested by its ability to promote the proliferation of 2E8 cells. Cell proliferation
was measured after 66 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the
growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.06 - 0.09 ng/mL.
(B) 1 μg of Human Recombinant IL-7 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by
Coomassie Blue staining. Human Recombinant IL-7 has a predicted molecular mass of 17.5 kDa.
