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概要
Interleukin 6 (IL-6) is a pleiotropic growth factor with the wide range of biological activities in immune regulation, hematopoiesis, and oncogenesis. IL-6 is produced by a variety of cell types including T cells, B cells, monocytes and macrophages, fibroblasts, hepatocytes, vascular endothelial cells, and various tumor cell lines. On its own or in combination with other factors such as IL-2 and interferon-γ, IL-6 stimulates the proliferation of B cells, T cells, and hybridoma cells (Hirano et al.; Mihara et al.; Tanaka et al). In combination with cytokines such as IL-3, GM-CSF and SCF, IL-6 has been shown to promote hematopoietic progenitor cell proliferation and differentiation in vitro. IL-6 signals through a cell surface type I cytokine receptor complex consisting of the ligand-binding IL-6α (CD126) and the signal-transducing gp130 subunits. The binding of IL-6 to its receptor system includes activation of JAK/STAT signaling pathway (Mihara et al.; Peters et al.; Tanaka et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IL-6 | 78050, 78050.1, 78050.2 | BX23155 and higher | English |
| Safety Data Sheet | Human Recombinant IL-6 | 78050, 78050.1, 78050.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IL-6 was tested by its ability to promote the proliferation of B9 cells. Cell proliferation
was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell
proliferation is at 50% of maximum. The EC50 in the above example is 3.31 pg/mL.
(B) 1 μg of Human Recombinant IL-6 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by
Coomassie Blue staining. Human Recombinant IL-6 has a predicted molecular mass of 21 kDa.
