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概要
Interleukin 5 (IL-5) is a member of the short-chain 4-α-helical bundle subset of hematopoietic cytokines. It binds to a receptor consisting of IL-5Ra, which is specific for IL-5R, and common beta chain, which is shared with the receptor for IL-3 and GM-CSF (Shearer). Upon binding to its receptor, IL-5 activates the JAK/STAT and MAPK pathways. IL-5 is produced by Th2 cells, eosinophils, and activated mast cells. It functions in the recruitment, activation, proliferation, and survival of eosinophils, thus playing an important role in allergic inflammation, asthma, and parasite immunity. Stimulation of eosinophils with IL-5 leads to their activation, upregulation of CD11b expression, and inhibition of apoptosis (Shearer).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IL-5 | 78048, 78048.1 | All | English |
| Safety Data Sheet | Human Recombinant IL-5 | 78048, 78048.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IL-5 was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation
was measured after 62 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the
growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.13 - 0.2 ng/mL.
(B) 1 μg of Human Recombinant IL-5 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by
Coomassie Blue staining. Human Recombinant IL-5 is a homodimer of 13.3 kDa subunits and has a predicted total molecular mass of
26.6 kDa.
