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概要
Human Interleukin 4 (IL-4) is important for immune responses to helminth infection as well as in allergic responses (Olpihant et al.). The IL-4 receptor consists of a heterodimer of IL-4Ra and common gamma chain. IL-4 receptor engagement leads to the activation of JAK1/3 and the recruitment of STAT6 and IRS1/2 (Nelms et al.). IL-4 drives immunoglobulin class switching in B cells (to IgE, IgG4), mast cell hyperplasia, mucus production, and the differentiation of naïve T cells into Th2 cells, which produce IL-4, IL-5, IL-10, and IL-13 (Bao et al.; Nelms et al.; Olpihant et al.). In addition to Th2 cells, IL-4 is produced by CD4+ NK T cells, γ/δ T cells, activated basophils, eosinophils, and mast cells. Human IL-4 does not cross-react with mouse cells (Park et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IL-4 | 78045, 78045.1, 78045.2, 78045.3 | All | English |
| Safety Data Sheet | Human Recombinant IL-4 | 78045, 78045.1, 78045.2, 78045.3 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IL-4 was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation
was measured after 72 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the
growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.16 - 0.24 ng/mL.
(B) 1 μg of Human Recombinant IL-4 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by
Coomassie Blue staining. Human Recombinant IL-4 has a predicted molecular mass of 15.1 kDa.
