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概要
Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang et al.; Dorssers et al.; Broughton et al.). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton et al.). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock et al.). This product is animal component-free.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IL-3, ACF | 78146, 78146.1, 78146.2 | All | English |
| Safety Data Sheet | Human Recombinant IL-3, ACF | 78146, 78146.1, 78146.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IL-3, ACF was tested by its ability to promote the proliferation of TF-1 cells. Cell
proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at
which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.244 ng/mL.
(B) 1 μg of Human Recombinant IL-3, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and
visualized by Coomassie Blue staining.
