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概要
Indian hedgehog (IHH) is one of three proteins in the hedgehog family which includes sonic hedgehog and desert hedgehog. Hedgehog proteins are important signaling molecules, acting through patched and smoothened receptors. During embryonic development, they are expressed in the fetal lung, gut, stomach, liver, kidney, and pancreas. IHH is strongly expressed in cartilage and regions of developing bone, and its signaling regulates growth and differentiation of bone tissue (Marigo et al.; Valentini et al.; St-Jacques et al.). IHH is also involved in yolk sac vasculogenesis, indicating a role in the differentiation of epiblast cells into endothelial and red blood cells (Byrd et al.). IHH induces the expression of parathyroid hormone-related peptide (PTHrP), which negatively regulates IHH during chondrocyte differentiation and proliferation (Vortkamp et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IHH | 78197, 78197.1 | All | English |
| Safety Data Sheet | Human Recombinant IHH | 78197, 78197.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IHH was tested by its ability to induce alkaline phosphatase activity by CCL-226 cells. Alkaline phosphatase activity was measured by a fluorometric assay method. The EC50 is defined as the effective concentration of the signaling molecule at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the example above is less than 3 μg/mL. (B) 2 μg of Human Recombinant IHH was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IHH has a predicted molecular mass of 19.7 kDa.
