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概要
Insulin-like growth factor 1 (IGF-I) is a polypeptide that belongs to the family of insulin-like growth factors that are similar in molecular structure to proinsulin. IGF-I binds to the IGF-I receptor and is a potent activator of the PI3K/AKT pathway and also activates ERK1/2 signaling. IGF-I is required for embryonic development, and it is produced mainly in the liver in response to a hepatocyte growth hormone. In the absence of insulin, IGF-I is necessary for the maintenance of human pluripotent stem cells (Wang et al.). Together with IL-3, IGF-I stimulates differentiation and proliferation of myeloid cells and has been shown to regulate lymphopoiesis by stimulating proliferation and differentiation of T and B cells in lymphoid organs (Heemskerk et al.). This product is animal component-free.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IGF-I, ACF | 78142, 78142.1, 78142.2 | All | English |
| Safety Data Sheet | Human Recombinant IGF-I, ACF | 78142, 78142.1, 78142.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IGF-I, ACF was tested by its ability to promote the proliferation of FDC-P1 cells. Cell proliferation was measured after 72 hours in culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.856 ng/mL. (B) 1 μg of Human Recombinant IGF-I, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.
