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细胞因子

Human Recombinant IFN-alpha 2B

Interferon-alpha 2B

概要
技术资料
数据及文献

概要

Interferon-alpha (IFN-α) is a type I interferon, produced by virus infected cells, and is released as a soluble factor to initiate antiviral responses (Isaacs & Lindenmann). IFN-α2 is the most potent IFN-α used in fundamental research and in most clinical applications. The best known IFN-α2 subvariants, 2A and 2B, differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (von Gabain et al.). Type I IFNs exert potent antitumor activity by increasing the cytotoxic activity of NK and T cells, as well as inhibiting the proliferation of cancer cells (Paul et al.). Additionally, it has been shown that proinflammatory IFN-α modulates the function of B cells in patients with systemic lupus erythematosus (Chang et al.) and pegylated form of IFN-α 2A and 2B has implications in the treatment of Hepatitis C (Foster et al.).

技术资料

Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet Human Recombinant IFN-alpha 2B 78077, 78077.1 All English
Safety Data Sheet Human Recombinant IFN-alpha 2B 78077, 78077.1 All English

数据及文献

Data

(A) The biological activity of Human Recombinant IFN-alpha 2B was measured with A549 cells exposed to the encephalomyocarditis (EMC) virus in a cytopathic effect (CPE) assay. Cell survival was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell survival is at 50% of maximum. The EC50 in the example above is 1.0 pg/mL. (B) 1 μg of Human Recombinant IFN-alpha 2B was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IFN-alpha 2B has a predicted molecular mass of 19.4 kDa.