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概要
Interferon-alpha (IFN-α) is a type I interferon, produced by virus-infected cells, and is released as a soluble factor to initiate antiviral responses (Isaacs & Lindenmann). IFN-α2 is the most potent IFN-α used in fundamental research and in most clinical applications. The best-known IFN-α2 subvariants, 2A and 2B, differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (von Gabain et al.). Type I IFNs exert potent antitumor activity by increasing the cytotoxic activity of NK and T cells, as well as by inhibiting the proliferation of cancer cells (Paul et al.). Additionally, it has been shown that proinflammatory IFN-α modulates the function of B cells in patients with systemic lupus erythematosus (Chang et al.), and pegylated forms of IFN-alpha 2A and 2B have implications in the treatment of hepatitis C (Foster et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant IFN-alpha 2A | 78076, 78076.1 | All | English |
| Safety Data Sheet | Human Recombinant IFN-alpha 2A | 78076, 78076.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant IFN-alpha 2A was measured with A549 cells exposed to the encephalomyocarditis (EMC) virus in a cytopathic effect (CPE) assay. Cell survival was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell survival is at 50% of maximum. The EC50 in the example above is 3.6 pg/mL. (B) 1 μg of Human Recombinant IFN-alpha 2A was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IFN-alpha 2A has a predicted molecular mass of 19.4 kDa.
