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概要
Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic growth factor that promotes proliferation, motility, survival, and differentiation. HGF binds and promotes the dimerization and activation of the receptor tyrosine kinase c-MET, and stimulates PI3K/AKT, FAK, JNK, and ERK1/2 signaling (Organ & Tsao). HGF stimulates migration of cells during embryogenesis, induces cell motility and scattering of epithelial cells, and regulates epithelial-mesenchymal transition. It is a chemoattractant for motor neurons, and regulates the development of sensory, sympathetic, parasympathetic, and cortical neurons as well as the proliferation of oligodendrocytes and glial development. Additionally, HGF is cytoprotective and regulates liver growth and regeneration (Nakamura et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant HGF | 78019, 78019.1, 78019.2 | All | English |
| Safety Data Sheet 1 | Human Recombinant HGF | 78019, 78019.1 | All | English |
| Safety Data Sheet 2 | Human Recombinant HGF | 78019, 78019.1, 78019.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant HGF was tested by its ability to promote the proliferation of 4MBr5 cells. Cell
proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at
which cell proliferation is at 50% of maximum. The EC50 in the above example is 7.2 ng/mL.
(B) 5 μg of Human Recombinant HGF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized
by Coomassie Blue staining. Human Recombinant HGF has a predicted molecular mass of 88 - 90 kDa (single chain), 59 - 61 kDa (alpha
chain), or 30 - 34 kDa (beta chain).
