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概要
GRO (growth-regulated oncogene)-beta or CXCL2 is a member of the CXC family, which plays an integral role in recruitment and activation of neutrophils and basophils in response to tissue injury and microbial infection. GRO-beta and GRO-gamma are closely related to GRO-alpha and share 90% and 86% amino acid sequence homology, respectively, with GRO-alpha. Receptor-binding studies have demonstrated that GRO-alpha, -beta, and -gamma signal mainly through G protein-coupled receptors CXCR1 and CXCR2 (Ahuja & Murphy). GRO-beta is expressed in epithelial cells, monocytes, fibroblasts, and melanocytes and is further induced during inflammatory, epithelialization, and angiogenic processes, for example during the wound healing process of human burn wounds (Zaja-Milatovic & Richmond). GRO-beta also stimulates mitogenesis in certain human melanoma cells (Unemori et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant GRO-beta (CXCL2) | 78112, 78112.1, 78112.2 | All | English |
| Safety Data Sheet | Human Recombinant GRO-beta (CXCL2) | 78112, 78112.1, 78112.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant GRO-beta (CXCL2) was tested by its ability to mobilize Ca2+ in CHO-K1/Gα15/hCXCR2 cells (human Gα15 and human CXCR2 stably expressed in CHO-K1 cells). Ca2+ mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the above example is less than 0.2 μg/mL. (B) 2 μg of Human Recombinant GRO-beta (CXCL2) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.
