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概要
GRO (growth-regulated oncogene)-alpha or CXCL1 is a member of the CXC family, which plays an integral role in recruitment and activation of neutrophils in response to tissue injury and microbial infection. GRO-alpha was initially identified by its growth stimulatory activity on malignant melanoma cells (Anisowicz et al.; Bechara et al.). GRO-alpha is closely related to GRO-beta (CXCL2), GRO-gamma (CXCL3), and interleukin 8 (CXCL8). Receptor-binding studies have demonstrated that GRO-alpha, -beta, and -gamma signal mainly through G protein-coupled receptor CXCR2 (Ahuja & Murphy). GRO-alpha is expressed in epithelial cells, monocytes, fibroblasts, and melanocytes, and is further induced during inflammatory, epithelialization, and angiogenic processes, such as during healing of human burn wounds (Zaja-Milatovic & Richmond). GRO-alpha, along with CXCL8, has been found to be critical for neutrophil mobilization and degranulation, as well as for vascular permeabilization and angiogenesis (Rudack et al.). GRO-alpha also stimulates mitogenesis in certain human melanoma cells (Unemori et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant GRO-alpha (CXCL1) | 78063, 78063.1 | All | English |
| Safety Data Sheet | Human Recombinant GRO-alpha (CXCL1) | 78063, 78063.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant GRO-alpha (CXCL1) was tested by its ability to mobilize Ca2+ in CHO-K1/Gα15/hCXCR2 cells (human Gα15 and human CXCR2 stably expressed in CHO-K1 cells). Ca2+ mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the example above is less than 0.1 μg/mL. (B) 2 μg of Human Recombinant GRO-alpha (CXCL1) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant GRO-alpha (CXCL1) has a predicted molecular mass of 7.8 kDa.
