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概要
Growth differentiation factor 11 (GDF-11) is a member of transforming growth factor beta (TGF-β) family. It binds to the TGF-β receptors ALK4, ALK5, and ALK7 and activates the SMAD signaling pathway (Ho et al.). GDF-11 regulates the development of the olfactory system, retina, and pancreas, as well as anterior/posterior patterning of the axial skeleton (Lee & Lee). GDF-11 is an endocrine factor expressed in skeletal muscle, brain, and dental pulp (Kondás et al.). Studies in mice showed that GDF-11 regulates muscle and cardiac aging, and stimulates neurogenesis by remodeling blood vessels (Katsimpardi et al.; Loffredo et al.; Sinha et al.). This product is animal component-free.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant GDF-11, ACF | 78189, 78189.1, 78189.2 | All | English |
| Safety Data Sheet | Human Recombinant GDF-11, ACF | 78189, 78189.1, 78189.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant GDF-11, ACF was tested by its ability to induce alkaline phosphatase activity in ATDC5 cells. Alkaline phosphatase activity was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the example above is 3.54 ng/mL. (B) 1 μg of Human Recombinant GDF-11, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant GDF-11, ACF has a predicted molecular mass of 24.9 kDa (12.5 kDa per monomer).
