网站首页
概要
Bone morphogenetic protein 2 (BMP-2) is a member of the transforming growth factor beta (TGF-β) superfamily. BMP-2 is a disulfide-linked homodimer, acts as a ligand for complexes of type I and II BMP receptors, and primarily activates SMAD1/5/8 signaling (Nohe et al.). BMP-2 is a potent differentiation factor and directs human pluripotent stem cells (hPSCs) towards various cell types including extra-embryonic endoderm, mesenchymal cells, and chondrocytes (Pera et al.). Although BMP-2 expression is low in healthy cartilage, its expression is upregulated at the site of cartilage damage (Blaney Davidson et al.). BMP-2 induces bone and cartilage formation in vitro and is able to induce chondrogenesis in human mesenchymal stem cells (Schmitt et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant BMP-2 | 78004, 78004.1, 78004.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant BMP-2 was tested by its ability to promote alkaline phosphatase production of ATDC-5
cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective
concentration of the growth factor at which alkaline phosphatase production is at 50% of maximum. The EC50 in the above example is
0.09 μg/mL.
(B) 2 μg of Human Recombinant BMP-2 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized
by Coomassie Blue staining. Human Recombinant BMP-2 has a predicted molecular mass of 26 kDa.
