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概要
B cell activating factor (BAFF) is part of the tumor necrosis factor (TNF) superfamily and is expressed by monocytes, macrophages, dendritic cells, and neutrophils (Mackay & Browning; Scapini et al.). It binds to 3 receptors: BAFF-R, TACI, and BCMA, all of which are expressed by B cells at various times in their development (Liu & Davidson). BAFF-R signaling activates the NF-kB signaling pathway, and promotes B cell survival, Ig class switching, and antigen presentation (Bossen & Schneider; Liu & Davidson; Naradikian et al.). BAFF also has functions in other cell types: it promotes monocyte survival and activation, proinflammatory cytokine secretion, and differentiation into macrophages (Chang et al.), and co-stimulates anti-CD3-mediated activation of human T cells (Huard et al.; Mackay & Leung).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant BAFF | 78002, 78002.1, 78002.2 | All | English |
| Safety Data Sheet | Human Recombinant BAFF | 78002, 78002.1, 78002.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant BAFF was tested by its ability to promote mitosis of RPMI-8226 cells. Mitogenic activity
was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which
mitogenic activity is at 50% of maximum. The EC50 in the above example is less than 20 ng/mL.
(B) 1 μg of Human Recombinant BAFF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions visualized by
Coomassie Blue staining. Human Recombinant BAFF has a predicted molecular mass of 17 kDa.
