网站首页
概要
Conveniently and consistently add transforming growth factor beta 1 (TGF-ꞵ1) to your cultures with this cell culture-ready formulation. This human recombinant TGF-ꞵ1 is precisely reconstituted to 0.1 mg/ml in 100 mM acetic acid and requires no additional preparation, improving reproducibility. Endotoxin levels are verified, using the LAL method to ensure consistency for use across multiple applications. A member of the TGF-β superfamily, TGF-β1 regulates diverse cellular phenotypes. TGF-β1 binds to serine-threonine kinase type I and II receptors and activates signal transduction through SMAD2/3 proteins.
TGF-β1 has been used for:
hPSC CULTURE MAINTENANCE
● Together with basic fibroblast growth factor (bFGF), supports the culture of undifferentiated human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells (Amit et al. Biol Reprod, 2004).
EXOGENOUS CELL SIGNALING
● Regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, and metabolism (Massagué Nat Rev Mol Cell Biol, 2000; McDowell et al. Curr Biol, 1997).
IMMUNE CELL HOMEOSTASIS
● Modulate B cell responses and mediate immunosuppressive effects on T cells and neutrophils (Letterio & Roberts Annu Rev Immunol, 1998).
技术资料
Scientific Resources
Product Documentation
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant TGF-beta 1 (Cell Culture Ready) | 100-0856 | All | English |
| Safety Data Sheet | Human Recombinant TGF-beta 1 (Cell Culture Ready) | 100-0856 | 全部 | English |
数据及文献
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
数据
Figure 1. Human Recombinant TGF-beta 1 Is Bioactive
The biological activity of Human Recombinant TGF-beta 1 was tested by its ability to induce luciferase expression in a SMAD/TGF luciferase reporter cell line. The EC50 is defined as the effective concentration of the agonist at which luminescence is 50% of maximum. The EC50 in the example above is 0.52 ng/mL. Normalized mean % of 3 replicate wells is shown for each data point. Each assay is calibrated with a Non-WHO International Reference Material (NIBSC code: 89/514). EC50 is assay-specific; titrations should be performed to determine the optimal concentration for the intended application.
Figure 2. Human Recombinant TGF-beta 1 Has High Purity
Human Recombinant TGF-beta 1 (1 μg) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie Blue staining. Human Recombinant TGF-beta 1 is a homodimer of 12.8-kDa subunits with a predicted molecular mass of 25.6 kDa.
