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概要
Sonic hedgehog (Shh) is a member of the Hedgehog family of secreted signaling proteins that has a prominent role in patterning during early mammalian development (Ho & Scott). It is expressed during early embryogenesis in a variety of tissues (Finco et al.). During patterning of the central nervous system, Shh directly acts on cells to specify neural cell fates, regulates proliferation and survival of oligodendrocyte precursors and neural crest cells and stimulates proliferation, differentiation, and axon growth (Ho & Scott; Martí). Shh also regulates T cell differentiation and activation (Crompton et al.). Recombinant Mouse Shh is a fully biologically active molecule.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Mouse Recombinant Shh | 78066, 78066.1, 78066.2 | All | English |
| Safety Data Sheet | Mouse Recombinant Shh | 78066, 78066.1, 78066.2 | All | English |
数据及文献
Data
(A) The biological activity of Mouse Recombinant Shh was tested by its ability to promote alkaline phosphatase production of CCL-226
cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective
concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.8 μg/mL.
(B) 2 μg of Mouse Recombinant Shh was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by
Coomassie Blue staining. Mouse Recombinant Shh has a predicted molecular mass of 19.8 kDa.
