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概要
Stromal cell-derived factor 1 beta (SDF-1 beta) is a splicing variant of CXCL12 and a member of the CXC family of chemokines. SDF-1 beta is constitutively and ubiquitously expressed in most tissues as SDF-1α (89 amino acids) and SDF-1β (93 amino acids) resulting from alternative gene splicing (Janowski). These variants regulate hematopoiesis, lymphocyte homing, B-lineage cell growth, and angiogenesis (De La Luz Sierra et al.). SDF-1 beta binds primarily to CXC receptor 4 (CXCR4, CD184); this receptor has also been shown to serve as a co-receptor for T cell-tropic HIV-1. The SDF-1 beta/CXCR4 axis is involved in tumor progression, angiogenesis, metastasis, and survival (Teicher & Fricker). SDF-1 beta is highly expressed in lymph nodes, lung, liver, and bone marrow under homeostatic conditions, and it modulates immune surveillance and development (Sánchez-Martín et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Mouse Recombinant SDF-1 beta (CXCL12) | 78101, 78101.1 | All | English |
| Safety Data Sheet | Mouse Recombinant SDF-1 beta (CXCL12) | 78101, 78101.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant SDF-1 beta (CXCL12) was tested by its ability to mobilize Ca2+ in CHO-K1/Gα15/mCXCR4 cells (human Gα15 and mouse CXCR4 stably expressed in CHO-K1 cells). Ca2+ mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the example above is less than 2.5 μg/mL. (B) 5 μg of Human Recombinant SDF-1 beta (CXCL12) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant SDF-1 beta (CXCL12) has a predicted molecular mass of 8.5 kDa.
