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概要
Leukemia inhibitory factor (LIF) is an interleukin 6 class cytokine that regulates a broad variety of developmental functions. After LIF binds to the LIF receptor (LIFR), LIFR associates with gp130 and activates JAK/STAT and MAPK signaling (Auernhammer & Melmed; Suman et al.). LIFR activation of STAT3 is essential for maintaining the mouse embryonic stem cell phenotype (Niwa et al.). Produced by the endometrium, LIF plays an important autocrine and paracrine role in implantation by regulating proliferation, invasion, and differentiation of trophoblasts following blastocyst attachment (Auernhammer & Melmed; Suman et al.). Human LIF can be used for the maintenance of mouse embryonic stem cells; however, mouse LIF cannot bind to the human receptor, thus rendering mouse LIF inactive (Dahéron et al.). LIF is produced by CD4+ and activated regulatory T cells, and promotes Foxp3 expression, while repressing Th17 lineage-specific genes (Metcalfe). LIF is also secreted by mesenchymal stromal cells, where it supports hematopoiesis and immune modulation (Nasef et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Mouse Recombinant LIF | 78056, 78056.1, 78056.2 | All | English |
| Safety Data Sheet | Mouse Recombinant LIF | 78056, 78056.1 | All | English |
数据及文献
Data
(A) The biological activity of Mouse Recombinant LIF was tested by its ability to promote the proliferation of M1 cells. Cell proliferation
was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell
proliferation is at 50% of maximum. The EC50 in the above example is 2.7 pg/ml.
(B) 2 μg of Mouse Recombinant LIF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by
Coomassie Blue staining. Mouse Recombinant LIF has a predicted molecular mass of 19.9 kDa.
