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概要
Interleukin 33 (IL-33) is a pro-inflammatory cytokine from the IL-1 family. It binds to the ST2 receptor and activates NF-kB and MAPK pathways. IL-33 is expressed by epithelial cells, smooth muscle cells, and fibroblasts in various tissues and organs, as well as resting basophils, mast cells, eosinophils, natural helper cells, group 2 innate lymphoid cells, dendritic cells, and activated macrophages (Schmitz et al.; Yasuda et al.). It contributes to allergic inflammation by stimulating production of the cytokines IL-4, IL-5, and IL-13 in Th2 cells, and stimulates host defense against microbial and viral infections (Liew; Yasuda et al.). In the central nervous system, IL-33 is produced by endothelial cells and astrocytes. It induces proliferation of microglia and mediates production of pro-inflammatory cytokines (Yasuoka et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Mouse Recombinant IL-33 | 78044, 78044.1 | All | English |
| Safety Data Sheet | Mouse Recombinant IL-33 | 78044, 78044.1 | All | English |
数据及文献
Data
(A) The biological activity of Mouse Recombinant IL-33 was tested by its ability to promote the proliferation of D10S cells. Cell
proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at
which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.47 ng/mL.
(B) 5 μg of Mouse Recombinant IL-33 was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue
staining. Mouse Recombinant IL-33 has a predicted molecular mass of 17.5 kDa.
