网站首页
概要
Interleukin 13 (IL-13) is a type I cytokine, and signals through type I cytokine receptors to activate JAK/STAT and IRS-1/IRS-2 pathways. IL-13 is produced by T cells and innate lymphoid cells (Pulendran & Artis) and it inhibits production of pro-inflammatory cytokines, prostaglandins, and reactive oxygen and nitrogen species by monocytes and macrophages (Hershey). Unlike human B cells, IL-13 has no effect on mouse B cell development and function. IL-13 promotes eosinophil survival, activation, and recruitment, and also activates mast cells (Hershey). IL-13 regulates gastrointestinal parasite expulsion, airway hyperresponsiveness, allergic inflammation, tissue eosinophilia, goblet cell hyperplasia, intracellular parasitism, tissue remodeling, tumor cell growth, and fibrosis. IL-13 induces hypercontractility of smooth muscles by directly acting on the muscle cells as well as the enteric nerves (Wynn).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Mouse Recombinant IL-13 | 78030, 78030.1 | All | English |
| Safety Data Sheet | Mouse Recombinant IL-13 | 78030, 78030.1 | All | English |
数据及文献
Data
(A) The biological activity of Mouse Recombinant IL-13 was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation
was measured after 66 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the
growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 2.3 - 3.4 ng/mL.
(B) 1 ug of Mouse Recombinant IL-13 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized
by Coomassie Blue staining. Mouse Recombinant IL-13 has a predicted molecular mass of 12.3 kDa.
