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概要
Interleukin 12 (IL-12p70) is a heterodimeric cytokine composed of p35 and p40 subunits. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils and B cells in response to bacterial products and cytokines such as IFN-γ. The IL-12 receptor is expressed on T, NK, and dendritic cells. Upon binding, IL-12 initiates signaling via the JAK/STAT signaling pathway and stimulates NK, B, and T cells to produce IFN-γ (Watford et al.). It also regulates cytokine synthesis, proliferation of T and NK cells, and stimulates differentiation of CD4+ and CD8+ T cells (Germann & Rüde). Mice that are deficient in IL-12 are susceptible to many intracellular pathogens and have impaired IFN-γ secretion, Th1 differentiation and NK cytolytic activity; however, Th2 development and IL-4 production are enhanced (Watford et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Mouse Recombinant IL-12 | 78028, 78028.1 | All | English |
| Safety Data Sheet | Mouse Recombinant IL-12 | 78028, 78028.1 | All | English |
数据及文献
Data
(A) The biological activity of Mouse Recombinant IL-12 was tested by its ability to induce SEAP in HEK-Blue™ IL-12 cells. The EC50 is defined as the effective concentration of the growth factor at which expression of CD25 is at 50% of maximum. The EC50 in the above example is 2.3 ng/mL. For a representative bioactivity plot of CD25 expression on CD8+ T cells, contact us at techsupport@stemcell.com.
(B) 1 μg of Mouse Recombinant IL-12 was resolved with SDS-PAGE under reducing (+) non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant IL-12 is a heterodimer comprising one p40 subunit and one p35 subunit. The total predicted molecular mass is 57.5 kDa but migrates to an apparent molecular mass of 70 kDa.
