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概要
Interleukin 7 (IL-7) is a member of the type I cytokine family that is critical for T and B cell development and survival. It is produced by non-hematopoietic cells in the thymus, lymphoid organs, and by bone marrow stromal cells (Lundström et al.). IL-7 binds to a receptor composed of common gamma chain and IL-7Ra (CD127) and signals through the JAK/STAT and PI3K pathways. IL-7 regulates the survival of naïve and memory CD4+ and CD8+ T cells, γδ T cells, NK T cells, innate lymphoid cells, and T regulatory cells (Carrette & Surh), and provides signals to promote survival, proliferation, and differentiation during B cell development (Clark et al.; Corfe & Paige).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Mouse Recombinant IL-7 | 78054, 78054.1 | All | English |
| Safety Data Sheet | Mouse Recombinant IL-7 | 78054, 78054.1 | All | English |
数据及文献
Data
(A) The biological activity of Mouse Recombinant IL-7 was tested by its ability to promote the proliferation of 2E8 cells. Cell proliferation
was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell
proliferation is at 50% of maximum. The EC50 in the above example is less than 0.4 ng/mL.
(B) 5 μg of Mouse Recombinant IL-7 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by
Coomassie Blue staining. Mouse Recombinant IL-7 has a predicted molecular mass of 15 kDa but migrates to an apparent molecular
mass of ~24 kDa.
