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概要
Interleukin 5 (IL-5) is a member of the short-chain 4-α-helical bundle subset of hematopoietic cytokines. It binds to the specific α receptor, which in turn recruits the common β receptor that initiates signaling via JAK/STAT and MAPK pathways. IL-5 is produced by Th2 cells, eosinophils, activated mast cells, and ILC2 cells. IL-5 affects the differentiation, activation, and survival of eosinophils, thus playing an important role in allergic inflammation, asthma, and parasite immunity. Stimulation of eosinophils with IL-5 leads to their activation, upregulation of CD11b expression, and inhibition of apoptosis (Shearer).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Mouse Recombinant IL-5 | 78049, 78049.1 | All | English |
| Safety Data Sheet | Mouse Recombinant IL-5 | 78049, 78049.1 | All | English |
数据及文献
Data
(A) The biological activity of Mouse Recombinant IL-5 was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation
was measured after 70 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the
growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.15 - 0.22 ng/mL.
(B) 1 μg of Mouse Recombinant IL-5 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by
Coomassie Blue staining. Mouse Recombinant IL-5 has a predicted molecular mass of 26.3 kDa.
