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概要
Transforming growth factor beta 1 (TGF-β1) is a member of the TGF-β superfamily and regulates diverse cellular phenotypes. TGF-β1 binds to serine-threonine kinase type I and II receptors and activates signal transduction through SMAD2/3 proteins, thus regulating a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, and metabolism (Massagué; McDowell et al.). During human embryogenesis, TGF-β1 is expressed by endothelial and hematopoietic tissues and acts as an endogenous autocrine growth regulator in those cells (Gatherer et al.). Together with basic fibroblast growth factor (bFGF), TGF-β1 supports the culture of undifferentiated human embryonic stem cells and induced pluripotent stem cells (Amit et al.). TGF-β1 is produced in latent form by many blood cells and is present in high amounts in platelets and bones. Once activated, TGF-β1 helps to maintain immune homeostasis by modulating B cell response and mediating immunosuppressive effects on T cells and neutrophils (Letterio & Roberts).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant TGF-beta 1 | 78067, 78067.1, 78067.2, 78067.3 | All | English |
| Safety Data Sheet | Human Recombinant TGF-beta 1 | 78067, 78067.1, 78067.2, 78067.3 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant TGF-beta 1 was tested by the ability to inhibit IL-4-induced HT-2 cell proliferation. Cell proliferation was measured after 2 days of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.17 ng/mL. (B) 1 μg of Human Recombinant TGF-beta 1 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.
