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概要
RANTES (regulated upon activation, normal T cell expressed and secreted), also known as CCL5, is a member of the CC family of chemokines and is able to recruit leukocytes to sites of inflammation (Schall et al.). RANTES is secreted by T lymphocytes, macrophages, platelets, synovial fibroblasts, tubular epithelium, and certain types of tumor cells (Aldinucci & Colombatti; Soria & Ben-Baruch). This chemokine exerts its effect by interacting with the chemokine receptors CCR1, CCR3, CCR4, and CCR5. RANTES plays an active role in recruiting a variety of leukocytes into inflammatory sites, including T cells, macrophages, eosinophils, and basophils. In collaboration with certain cytokines that are released by T cells such as IL-2 and IFN-γ, RANTES also induces the activation and proliferation of NK cells to generate CC chemokine-activated killer cells, which are highly cytolytic (Lv et al.; Maghazachi et al.). It has been shown that RANTES produced by CD8+ T cells inhibits HIV infection of primary human peripheral blood mononuclear cells (Appay & Rowland-Jones; Cocchi et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant RANTES (CCL5) | 78099, 78099.1 | All | English |
| Safety Data Sheet | Human Recombinant RANTES (CCL5) | 78099, 78099.1 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant RANTES (CCL5) was tested by its ability to induce chemotaxis of THP-1 cells. Cell migration was measured after 45 min using a fluorometric assay method. Increase in migration over basal level was seen starting at 10 ng/mL. (B) 1 μg of Human Recombinant RANTES (CCL5) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant RANTES (CCL5) has a predicted molecular mass of 7.9 kDa.
