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概要
The EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit is designed to isolate CD138+ (syndecan-1) cells from whole blood and bone marrow by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD138 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD138 antigen is expressed on normal and malignant plasma cells (but not mature B cells).
This product replaces the EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit (Catalog #18387) for even faster cell isolations.
This product replaces the EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit (Catalog #18387) for even faster cell isolations.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II | 17887 | All | English |
| Product Information Sheet | RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II | 17887RF | All | English |
| Safety Data Sheet 1 | EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II | 17887 | All | English |
| Safety Data Sheet 2 | EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II | 17887 | All | English |
| Safety Data Sheet 3 | EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II | 17887 | All | English |
| Safety Data Sheet 1 | RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II | 17887RF | All | English |
| Safety Data Sheet 2 | RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II | 17887RF | All | English |
| Safety Data Sheet 3 | RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II | 17887RF | All | English |
数据及文献
Data
Starting with fresh whole blood spiked with a multiple myeloma cell line, U266, the CD138+ cell content of the selected fraction typically ranges from 83.7 - 98.3%. In the above example, the purities of the start and final isolated fractions are 9.1% and 90.4%, respectively. NOTE: For samples with CD138+ starting frequency < 10 - 15%, the CD138+ purity of the isolated fraction may be variable. NOTE: Red blood cells were removed from the start sample by lysis prior to flow cytometry.
