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概要
The EasySep™ Human Plasmacytoid DC Isolation Kit is designed to isolate plasmacytoid dendritic cells (pDCs) from human peripheral blood mononuclear cells (PBMCs) or leukapheresis by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
This product replaces the EasySep™ Human Plasmacytoid DC Enrichment Kit (Catalog #19062), for even faster cell isolations and is compatible with a greater number of EasySep™ magnets.
This product replaces the EasySep™ Human Plasmacytoid DC Enrichment Kit (Catalog #19062), for even faster cell isolations and is compatible with a greater number of EasySep™ magnets.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Plasmacytoid DC Isolation Kit | 17977 | All | English |
| Product Information Sheet | RoboSep™ Human Plasmacytoid DC Isolation Kit | 17977RF | All | English |
| Safety Data Sheet | EasySep™ Human Plasmacytoid DC Isolation Kit | 17977 | All | English |
| Safety Data Sheet | RoboSep™ Human Plasmacytoid DC Isolation Kit | 17977RF | All | English |
数据及文献
Data
Figure 1. Typical EasySep™ Human Plasmacytoid DC Isolation Profile
Starting with leukapheresis samples, the pDC content (Lin-HLA-DR+CD123+) of the isolated fraction is typically 90 ± 5.3% (mean ± SD using the silver “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 0.2% and 89.8%, respectively.
