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概要
The EasySep™ Human Pan-ILC Enrichment Kit is designed to enrich Group 1, 2, and 3 Innate Lymphoid Cells (ILC1, 2, and 3) from leukapheresis products by immunomagnetic negative selection. Unwanted cells are targeted for removal by Tetrameric Antibody Complexes directed against non-ILCs and dextran-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as enriched ILCs are simply poured off into a new tube. Isolated cells are immediately available for flow sorting and other downstream applications.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Pan-ILC Enrichment Kit | 17975 | All | English |
| Product Information Sheet | RoboSep™ Human Pan-ILC Enrichment Kit | 17975RF | All | English |
| Safety Data Sheet | EasySep™ Human Pan-ILC Enrichment Kit | 17975 | All | English |
| Safety Data Sheet | RoboSep™ Human Pan-ILC Enrichment Kit | 17975RF | All | English |
数据及文献
Data
Figure 1. Typical EasySep™ Human Pan-ILC Enrichment Profile
Starting with fresh leukapheresis samples, the total ILC content (Lin-CD45+CD127+) of the enriched fraction typically ranges from 17 - 85%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.09% and 45% (or 0.1% and 54% of CD45+ cells), respectively.
