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概要
The EasySep™ Human Pan-Granulocyte Enrichment Kit is designed to isolate granulocytes (eosinophils, basophils and neutrophils) from normal peripheral whole blood by negative selection. Undesired cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-granulocytes and magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Pan-Granulocyte Isolation Kit | 19259 | All | English |
| Product Information Sheet | RoboSep™ Human Pan-Granulocyte Isolation Kit | 19259RF | All | English |
| Safety Data Sheet | EasySep™ Human Pan-Granulocyte Isolation Kit | 19259 | All | English |
| Safety Data Sheet | RoboSep™ Human Pan-Granulocyte Isolation Kit | 19259RF | All | English |
数据及文献
Data
Figure 1. Typical EasySep™ Human Pan-Granulocyte Isolation Profile
Starting with whole peripheral blood, the total granulocyte content of the isolated fraction typically ranges from 97 - 99%. As shown in the examples above, neutrophils are typically CD66b+CD16+. Eosinophils are typically CD66b+CD16- and basophils are CD66b- and can be further defined as CD203c+CD123+ and IgE+ (not shown).
