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概要
The EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit isolates highly purified human extracellular vesicles from plasma/serum and cell culture conditioned medium by immunomagnetic positive selection. Extracellular vesicles are labeled with Tetrameric Antibody Complexes recognizing CD9, CD81, and CD63, and magnetic particles. Labeled extracellular vesicles are separated without columns using an EasySep™ magnet. Unwanted biofluid components are simply poured off, while desired extracellular vesicles remain in the tube. Isolated extracellular vesicles are available for downstream applications such as DNA/RNA extraction, Western blot, or mass spectometry.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit | 17891 | All | English |
| Special Protocol | EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit | 17891 | All | English |
| Safety Data Sheet 1 | EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit | 17891 | All | English |
| Safety Data Sheet 2 | EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit | 17891 | All | English |
数据及文献
Data
Figure 1. Typical Western Blot Analyses of EVs Isolated from Human Plasma and Mesenchymal Stromal Cell (MSC)-Conditioned Medium
The Western blot analyses in the above examples show positive isolation of EVs with CD9, CD63, and CD81 tetraspanin markers from (A) human plasma and (B) MSC-conditioned medium. Western blots were run under non-reducing conditions.
