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概要
The EasySep™ Human Myeloid Positive Selection Kit II is designed to isolate CD33+ and CD66b+ cells from ammonium chloride lysed blood or bone marrow by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33, CD66b and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 and CD66b antigens are expressed on myeloid progenitors monocytes and granulocytes.
This product replaces the EasySep™ Human Myeloid Positive Selection Kit (Catalog #18653) for even faster cell isolations.
This product replaces the EasySep™ Human Myeloid Positive Selection Kit (Catalog #18653) for even faster cell isolations.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Myeloid Positive Selection Kit II | 17893 | All | English |
| Product Information Sheet | RoboSep™ Human Myeloid Positive Selection Kit II | 17893RF | All | English |
| Safety Data Sheet 1 | EasySep™ Human Myeloid Positive Selection Kit II | 17893 | All | English |
| Safety Data Sheet 2 | EasySep™ Human Myeloid Positive Selection Kit II | 17893 | All | English |
| Safety Data Sheet 3 | EasySep™ Human Myeloid Positive Selection Kit II | 17893 | All | English |
| Safety Data Sheet 1 | RoboSep™ Human Myeloid Positive Selection Kit II | 17893RF | All | English |
| Safety Data Sheet 2 | RoboSep™ Human Myeloid Positive Selection Kit II | 17893RF | All | English |
| Safety Data Sheet 3 | RoboSep™ Human Myeloid Positive Selection Kit II | 17893RF | All | English |
数据及文献
Data
Starting with peripheral blood MNCs, the myeloid cell content (CD14+CD66b+) of the isolated fraction is typically 96.5 ± 1.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 59.2% and 94.6%, respectively.
